Bio-Surfactant Formulation Guide Part 1 - Hard Surface Cleaning
What is a Biosurfactant?
Biosurfactants are amphiphilic compounds typically produced by yeasts and bacteria, which are secreted into the aqueous fermentation media in which the cells are growing. Biosurfactants possess the characteristic property of reducing the surface and interfacial tension, using the same mechanisms as chemical surfactants. In short, biosurfactants are a class of surfactants that are produced by yeasts and bacteria via fermentation.
Hard Surface Cleaner
Utilising Sophorolipids in hard surface cleaners allows a formulator to add a powerful non-ionic cleaner offering improved cleaning and mildness, with the knowledge that they are using a green sustainable cleaning ingredient; this product has no CLP grading.
Figure 1 example hard surface cleaner formulated with HoneySurf LF

Figure 2 example hard surface cleaner formulation with HoneySurf LF

How to formulate…
- Add half of the water to the vessel and Stir
- Add the Citric Acid and dissolve until a clear liquid - add Sodium Hydroxide to adjust to pH 10.4
- Add Amine Oxide AO10-30,
- Add Sophorolipids - adding this will make the mixture go cloudy, this will clear after a few minutes
- Add Dequest 2066
- Add the rest of the water and stir until clear - pH at this point will be about pH 10.4
- Adjust with Sodium Hydroxide to pH 10.4
- Finally add colour and perfume as desired
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